Noviogel is an improved synthetic hydrogel platform for cell studies, because they combine the unique benefits of both natural and synthetic biomaterials. Noviogel mechanically mimic collagen, while possessing the reproducibility and tunability of synthetic hydrogels. The porous fibrous polymer network has a stiffness that increases under applied force similar to collagen or fibrin. Noviogel is fully reversible thermo-sensitive which allows for ease of cell/tissue recovery and - downstream processing after culturing. By adding peptide sequences or growth factors, the hydrogels can be readily functionalized for different cell types. The unique combination of tunable bio functionality and biomechanics of the Noviogels makes them excellent matrices for 3D stem cell culture or regenerative medicine.
Noviogel is a new class of advanced polymers. The complex chemistry of the polymer allows the organization into a helix-like structure that is similar to the conformation of collagen, abundantly present in the extracellular matrix (structure around cells). Noviogel is an improved platform for cell studies, because it combines the unique benefits of natural and synthetic biomaterials. Noviogels uniquely perform like collagen, while possessing the same characteristics as other commercial hydrogels. Furthermore, it is fully reversible thermo sensitive, cells can be easy recovered and downstream processing after culturing is straightforward. The unique combination of tunable biofunctionality and biomechanics of the Noviogels makes them excellent matrices for 3D stem cell culture or regenerative medicine. In addition, Noviogel has a strain stiffness that increases under applied force, thereby enabling hydrogel strain stiffness modulation according to the needs of each cell type. Hereby, Noviogel creates a cellular microenvironment with the right mechanical cues to control cell expansion and differentiation.
Kouwer PHJ, Koepf M, Le Sage VAA, Jaspers M, van Buul AM, Eksteen-Akeroyd ZH, et al. Responsive biomimetic networks from polyisocyanopeptide hydrogels. Nature 2013.
Jaspers M, Dennison M, Mabesoone MFJ, MacKintosh FC, Rowan AE, Kouwer PHJ. Ultra-responsive soft matter from strain-stiffening hydrogels. Nat Commun. 2014.
Das RK, Gocheva V, Hammink R, Zouani OF, Rowan AE. Stress-stiffening-mediated stem-cell commitment switch in soft responsive hydrogels. Nat Mater 2015.
Bruekers, SMC, Jaspers M, Hendriks JMA, Kurniawan NA, Koenderink GH, Kouwer PHJ, Rowan AE, Huck WTS. Fibrin/fiber architecture influences cell spreading and differentiation. Cell Adhesion & Migration 2016.
Jaspers M, Pape ACH, Voets IK, Rowan AE, Portale G, Kouwer PHJ. Bundle formation in biomimectic hydrogels. Biomacromolecules 2016.
Zinkevich T, Venderbosch B, Jaspers M, Kouwer PHJ, Rowan AE, van Eck ERH, Kentgens APM. Solid state NMR characterization of tri-ethyleneglycol grafted polyisocynopeptides. Magn. Reson. Chem. 2016.
Mihaila S, Rowan AE, Feitz, WF, Oosterwijk E. Matrix-stiffness Driven Osteogenic Differentiation of Human Adipose Derived Stem Cells, TERMIS 2015.
Sun W, Eksteen-Akeroyd ZH, Nagelkerke A, Geutjes P, Zhou L, Wissing T, Wilson C, Feitz WF, Rowan AE, Oosterwijk E. Novel Polyisocyanopeptide Hydrogels for Rapid Vasculogenesis. TERMIS 2015.
Jaspers M et al. Nonlinear mechanics of hybrid polymer networks that mimic the complex mechanical environment of cells. Nat Commun. 2017 May 25;8:15478.
Deshpande SR et al.Biomimetic Stress Sensitive Hydrogel Controlled by DNA Nanoswitches. Biomacromolecules. 2017 Oct 9;18(10):3310-3317.
Hammink R et al.Affinity-Based Purification of Polyisocyanopeptide Bioconjugates. Bioconjug Chem. 2017 Sep 15
Zimoch, J., et.al. Polyisocyanopeptide hydrogels: a novel thermo-responsive hydrogel re-vascularization and the development of organotypic structures, Acta Biomaterialia (2018)
Nehar Celikkin et al. 3D Printing of Thermoresponsive Polyisocyanide (PIC) Hydrogels as Bioink and Fugitive Material for Tissue Engineering Polymers 2018, 10, 555