syntrix-P kit

Quantity: 10 ml
Catalog number: NCS01
€ 399,00
Syntrix-P is a synthetic matrix that can be used to create defined 3D micro-environments for a variety of cell-based assays. Syntrix-P has been functionalized with oligoethyleneglycol moieties, thereby presenting a low-adhesion surface to cells.

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Description

Syntrix-P is a synthetic matrix that can be used to create defined 3D micro-environments for a variety of cell-based assays. Syntrix-P has been functionalized with oligoethyleneglycol moieties, thereby presenting a low-adhesion surface to cells. In the presence of cell culture medium, Syntrix-P self-assembles into a hydrogel that exhibits a nanofiber structure, resembling the natural extracellular matrix. The resulting hydrogel has been found to support various cell lines (e.g. 3T3, HepG2, MDCK, and CaCo-2) and primary cells (e.g. HUVEC, hADSC and hESC). In contrast to currently used natural ECM products, it is well-defined and has very low batch-to-batch variation, offering a significant advantage for assay reproducibility. In contrast to Basement Membrane Extracts, Syntrix-P does not contain any cytokines, growth factors or other undefined bioactive materials, which enables scientists to work in a completely defined environment. Thus, the growth of the cells can be fine-tuned as supplements used to enhance cell performance such as growth factors can be added independently from the matrix to generate the optimal microenvironment for a given assay. Syntrix-P is very flexible in its applications. Certain applications require harder or softer matrices. The stiffness of Syntrix-P gel depends on the concentration of Syntrix-P used. Softer gels are made by lowering the concentration of Syntrix-P. Using the tables below the storage modulus (stiffness) of Syntrix-P can be varied in a range from approximately 200-2000 Pa. Gels of varying stiffness are prepared according to the procedure described in the Instruction Guide for use of Syntrix-P Kit, but with different dilutions of the various solutions in the Syntrix-P Kit. The dilutions required are listed in the Instruction Guide.

Instruction "How to prepare Syntrix hydrogels"



Downloads

Description Name Download link
Instruction Guide for Syntrix-P kit. Instruction Guide Syntrix-P Download
Recommendations for preparing different dilutions of Syntrix-P, staining protocols and more Syntrix-P protocols Download

References


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