Syntrix is a synthetic hydrogel technology based on the programmed self assembly of peptide-modified cyclohexane triacid derivatives into self-assembled nanofibers. Hydrogels based on this technology self-assemble rapidly and spontaneously under physiological conditions from appropriate precursor solutions, allowing mild encapsulation of cells.
A key feature of Syntrix is the ease and accuracy with which the surface of the fibers can be functionalized to present (biological) ligands. Mixing of individual hydrogel-ligand conjugates provides a single-step modular approach to generate diverse synthetic bioactive nanogel matrices approaching the complexity of biological hydrogel-like systems such as the natural ECM. By choosing bioactive peptides comprising cell-binding or other bioactive epitopes of ECM and other proteins, Noviocell’s Syntrix hydrogel technology provides control of the cell adhesive and instructive properties beyond what is possible for traditional synthetic biomaterials.
Since the nanofibers forming the hydrogel are held together only by non-covalent interactions between the monomers, encapsulation of cells takes place under mild conditions without a need for potentially cell-damaging reagents. Cellular material can be released using a pH trigger to disassemble the gels. Since the gels are made of low molecular weight components, they show excellent biodegradability.
Materials based on Noviocell’s Syntrix technology are biodegradable and has been proven to be compatible with the culture of a wide range of cell lines, primary cells, stem cells, organoids, and tumor organoids as well as to be able to direct epithelial organization.
L.M. Nibourg et al., Acta Ophthalmol.. 2016, 94, 721-729
L.M. Nibourg et al., Exp. Eye. Res. 2016, 143, 60-67
L. Ikonen et al., Biomed Research International. 2013, Article ID 285678
I. Kusters et al., PLoS ONE 2011, 6(5): e20435
A. Brizard et al., Angewandte Chemie, International Edition 2008, 47, 2063 – 20
F. Li et al. ChemPhysChem 2010, 11, 1956-196
M.R. de Jong et al., J. Control. Release, 132: e26-e27 (2008)
M. Montalti et al., Langmuir, 2006, 22, 2299 – 2303
K. J. C. van Bommel et al., Organic and Biomolecular Chemistry, 2005, 3, 2917 – 2920
M. De Loos et al., Org. Biomol. Chem. 2005, 3, 1631-1639
K.J.C. van Bommel et al. J. Control. Release 2005, 101, 287-290
A. Friggeri et al., Chemistry, a European Journal, 2005, 11, 5353 – 5361
A. Friggeri et al., Cosmetics & Toiletries 2005, 120: 67 – 76
K. J. C. van Bommel et al., Angewandte Chemie, International Edition 2004, 43, 1663 – 1667
A. Friggeri et al. J. Control. Release 2004, 97, 241-248
A. Heeres et al., Journal of the American Chemical Society, 2003, 125, 14252 – 14253